Reporter
no ATG GFP

Part:BBa_K579000

Designed by: Reo Yoshimura,Toshihiro Matsunami,Takahiro Nakagawa,Jyunpei Yokoigawa   Group: iGEM11_KIT-Kyoto   (2011-10-05)


no ATG GFP

This plasmid vector was designed to fuse GFP to C-terminal regions of any proteins.(We use BBa_E0240.) In our projects, we use this plasmid to fuse GFP to dMLF, DIAP2, API2-MALT1 and others to monitor expression of these proteins in E. coli and Drosophila.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 641


[edit]
Categories
//function/reporter/fluorescence
Parameters
protein